Are you interested in Cancer Research and want to do a PhD at the Garvan? Then come along to the open day and where you can meet with all the supervisors, discover some amazing projects, and get all the information you need to start your career in Cancer Research. Information Date: Wednesday 10th of September 2014 Time: 1.45-5pm […]Read More 2014 Garvan Cancer Division PhD Open Day, 10th September !
UPDATE: ImpactStory is not free, as I first thought, they currently have a 30 day free trail, then the cost is $45/year. Its clear that judging a researchers output purely on the impact/quality of the journal they do/don’t publish is not always the best way to accurately judge individual achievement and output. To that end, […]Read More ImpactStory: A new way to track individual article metrics
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]Read More Immunofluorescence Guide
General Guidelines These guides are written primarily for HeLa cells, but it should be possible to extend these to other cell lines with a bit of optimisation. 1) Make sure your cells are happy ! 2) Aim for around 75-85% confluence at the time of release. 3) Try and keep everything close to 37ºC, including […]Read More How to Synchronise Mammalian Cells in Culture
Grow cells in DMEM (Sigma #D9443) without L-Arg, L-Leu, L-Lys ! This DMEM is low glucose 1000mg/L so you will need to add and extra 3000mg/L to make it unto 4000mg/L in total. Thus 15ml of the 10% Glucose solution is needed for 500ml of DMEM. Need to add 50ml of Mass Spec Grade Serum […]Read More HeLa SILAC conditions
A complete ‘ish’ guide to Flow cytometry or FACS for cell cycle work. Covers all aspects from tissue culture, through to running and analyzing your samples on a FACScan or FACS Calibur. It was written by me a few years ago and so is a bit out of date. For example there is a new […]Read More Complete Guide to Cell Cycle based Flow Cytometry
Taken from Addgene’s guidelines [Link] Antibiotic Concentrations The following list shows recommended antibiotic concentrations for LB media or agar plates. Antibiotic Concentration Ampicillin 100 μg/mL Bleocin 5 μg/mL Carbenicillin 100 μg/mL Chloramphenicol 25 μg/mL Coumermycin 25 μg/mL Gentamycin 10 μg/mL Kanamycin 50 μg/mL Spectinomycin 50 μg/mL Tetracycline 10 μg/mL Example: To make 100 mL of LB/ampicillin […]Read More Antibiotic Concentrations for Bacterial Work
With the later versions of Photoshop CS4 and CS5 extended it is now very easy to add a scale bar to your microscope images. But before we go ahead you will need some information first. 1) The actual Pixel size of the camera attached to your microscope (here is a short list of some common cameras). […]Read More Adding a Scale Bar to Microscope Images