Taken from Addgene’s guidelines [Link]
The following list shows recommended antibiotic concentrations for LB media or agar plates.
Example: To make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB.
Unless otherwise indicated, the antibiotic powder can be dissolved in H20. Addgene recommends making 1000X stock solutions and storing aliquots at -20oC.
|Ampicillin||EMD Chemicals #171254|
|Bleocin||EMD Chemicals #203408|
|Carbenicillin||EMD Chemicals #205805|
|Chloramphenicol||EMD Chemicals #220551 (dissolve with EtOH)|
|Coumermycin||Sigma #C9270 (dissolve with DMSO)|
|Gentamycin||EMD Chemicals #345814|
|Kanamycin||EMD Chemicals # 420311|
|Spectinomycin||EMD Chemicals # 567570|
|Tetracycline||EMD Chemicals # 583411|
Please note that the catalog numbers given in the list above are only examples, and there are many additional companies that supply these reagents.