How to analyse single cells time-lapse movies using Fiji and/or Photoshop

In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse and track individual cells to generate single cell fate maps. Note, unless you are also planning on quantifying fluorescent intensity data in your movies, I strongly suggest converting all of your image files to high quality JPEGs or PNGs as this will significantly reduce file size and aid in downstream analysis.

This method is time consuming but the amount and quality of data you can generate from a single movie is enormous. It also has the added benefit that by looking at what your cells are doing you end up learning an and seeing things you would never see by other static methods.

Download full Powerpoint Tutorial NB: File size is ~110Mb and contains embedded videos

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