Very excited to announce that in collaboration with the very talented Dr Liz Caldon, I have published a full guide and protocol for the simple, yet very powerful method for tracking and analysing individual single cells from time-lapse movies. This method can generate a huge amount of quantitative data and give amazing insights into the mechanisms driving various phenotypes and responses to chemotherapy, gene silencing/knockdown and over-expression experiments, just to name a few. We hope that you enjoy it and find it useful.
Caldon, C., Burgess, A. (2019). Label Free, Quantitative Single-Cell Fate Tracking of Time-Lapse Movies MethodsX 6(), 2468-2475. https://dx.doi.org/10.1016/j.mex.2019.10.014