Great News, we have a new review article that has just been published online today in Inside the Cell! Its Open Access, so that means its free for everyone to read! During mitotic exit, phosphatases reverse thousands of phosphorylation events in a specific temporal order to ensure that cell division occurs correctly. This review explores how […]Read More New Review Article Published!! “Mechanisms Regulating Phosphatase Specificity During Mitotic Exit”
Great news we are currently looking for a new honours student for 2016. The title of the project is “Developing novel biosensors to monitor DNA damage in cancer cells”. Its a very exciting new project incorporating cutting edge microscopy and fluorescent biosensors. If you think you have what it takes and are interested please feel […]Read More Position available: 2016 Honours Student Project in our Lab
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or […]Read More Using ImageJ to Measure Cell Fluorescence
Great news, we recently published a collaborative paper in the journal Cell Division with the Lab of Dr Liz Caldon here at the Garvan. The title of the paper is “Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells”, and you can find it online here It also marked the […]Read More Congratulation to Sam our PhD student on his 1st Author Publication!
Great news, our latest article in @CellCycleJ is now online. “Degrading Claspin away with Cdh1 and Cyclin A. Cell Cycle (2015)” You can view the pre-print version here The News and Views article is based on the recent publication by Oakes, V. et al. entitled “Cyclin A/Cdk2 regulates Cdh1 and claspin during late S/G2 phase of […]Read More Latest News and Views Article now Online “Degrading Claspin away with Cdh1 and Cyclin A”.
Here is a recent talk I gave to some members of the public at the Garvan Institute of Medical Research. It is a very general and simple over-view of explaining 1) how cells in your body proliferate, 2) how this goes wrong in cancer, 3) the challenges we are facing in treating and killing cancer, and […]Read More Public Talk “Killing Cancer One Cell at a Time ” now on YouTube
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]Read More Immunofluorescence Guide
General Guidelines These guides are written primarily for HeLa cells, but it should be possible to extend these to other cell lines with a bit of optimisation. 1) Make sure your cells are happy ! 2) Aim for around 75-85% confluence at the time of release. 3) Try and keep everything close to 37ºC, including […]Read More How to Synchronise Mammalian Cells in Culture
A complete ‘ish’ guide to Flow cytometry or FACS for cell cycle work. Covers all aspects from tissue culture, through to running and analyzing your samples on a FACScan or FACS Calibur. It was written by me a few years ago and so is a bit out of date. For example there is a new […]Read More Complete Guide to Cell Cycle based Flow Cytometry
Great news, Cell Division Lab will be at the Sydney Light Optical Users Meeting, hosted by Dr Pamela Young at Sydney University, this Thursday (24th of July). I will be presenting a short seminar on “Imaging and Analysing Cell Division”. If you would like to attend please contact Pamela asap. Her details are below! Hope […]Read More We will be at the Sydney Light Optical Users Meeting on July 24th 2014