Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image manipulationRead More User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator
8th Garvan Signalling Symposium Date: Monday 31st of October and Tuesday 1st of November 2016 Venue: The Garvan Institute of Medical Research, Sydney Registration – Abstract submission to 15th of September 2016 The Garvan International Signalling Symposium is a premier meeting focused on the mechanisms of signal transduction. It began as a small meeting organised by […]Read More 8th Garvan Signalling Symposium – Registration Now Open !!!
Great news we are currently looking for a new honours student for 2016. The title of the project is “Developing novel biosensors to monitor DNA damage in cancer cells”. Its a very exciting new project incorporating cutting edge microscopy and fluorescent biosensors. If you think you have what it takes and are interested please feel […]Read More Position available: 2016 Honours Student Project in our Lab
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use Thresholds in Image J, and here are a few that are well worth checking out [Link1][Link2]. Below are some of the Basic Steps for using Thresholds: Open your image and […]Read More Using Thresholds to Measure and Quantify Cells in Image J
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or […]Read More Using ImageJ to Measure Cell Fluorescence
Great news, we recently published a collaborative paper in the journal Cell Division with the Lab of Dr Liz Caldon here at the Garvan. The title of the paper is “Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells”, and you can find it online here It also marked the […]Read More Congratulation to Sam our PhD student on his 1st Author Publication!
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]Read More Immunofluorescence Guide
Some more good news to coincide with today’s official release of our manuscript, one of our images has been chosen to be the feature image on the front cover. It’s a great honour, one that I am very proud of, and is the first time I have ever had a front cover ! You can […]Read More Were the front cover feature image on this months issue of Cell Cycle !
Here is a slide that I made and often use at the beginning of my talks to briefly explain what mitosis is. The images are taken from a live cell that we have labelled with fluorescent probes to highlight the DNA (red) and microtubules (green). Enjoy !Read More A brief introduction to Mitosis
Here is one of the images that we took using a Leica SP8 confocal microscope this week in the lab. It is a 3D image of a HeLa cell that has completely stuffed up mitosis (undergone mitotic catastrophe). It has separated whole chromosomes randomly into 2 daughter cells instead of separating the two identical chromatids in […]Read More Cell Image of the Week – Mitotic Catastrophe