DNA replication

New Co-Author Publication! Ensa controls S-phase length by modulating Treslin levels.

Posted on

Great news, we have published a co-authored paper entitled ‘Ensa controls S-phase length by modulating Treslin levels’ in the prestigious journal ‘Nature Communications’. This work was started back in 2011 when I was a Post-Doc in the laboratory of Anna Castro in France. It’s exciting to see those inital discoveries transition into the finished paper.
The article is Open Access and free to download, which you can do so here: http://www.nature.com/articles/s41467-017-00339-4

 

ABSTRACT

The Greatwall/Ensa/PP2A-B55 pathway is essential for controlling mitotic substrate phos- phorylation and mitotic entry. Here, we investigate the effect of the knockdown of the Gwl substrate, Ensa, in human cells. Unexpectedly, Ensa knockdown promotes a dramatic extension of S phase associated with a lowered density of replication forks. Notably, Ensa depletion results in a decrease of Treslin levels, a pivotal protein for the firing of replication origins. Accordingly, the extended S phase in Ensa-depleted cells is completely rescued by the overexpression of Treslin. Our data herein reveal a new mechanism by which normal cells regulate S-phase duration by controlling the ubiquitin-proteasome degradation of Treslin in a Gwl/Ensa-dependent pathway.

Advertisements

Position available: 2016 Honours Student Project in our Lab

Posted on Updated on

Great news we are currently looking for a new honours student for 2016.

The title of the project is “Developing novel biosensors to monitor DNA damage in cancer cells”.

Its a very exciting new project incorporating cutting edge microscopy and fluorescent biosensors.

If you think you have what it takes and are interested please feel free contact myself, or UNSW SoMS.
For more information on the UNSW honours program please visit: http://medicalsciences.med.unsw.edu.au/students/soms-honours/

Below is an example of the images that will be created during the project.

Cell Brains – Image of the Week

Image Posted on

Here is a HeLa cell in the first stages of mitosis (prophase) just as it has begun to condensing its DNA into chromosomes. At this point it looks strikingly similar to the folds of a human brain. The image has been put through a few photoshop filters to give this stunning final image.Cell-Brains-Colour-web

3rd Cell Cycle and Cancer Conference – April 2013 Montpellier France !

Quote Posted on Updated on

There is an excellent Conference coming up in the beautiful southern French city of Montpellier. There is an amazing line up of world class speakers, so book the dates in your calendar for 2-5th April 2013 !

Closing date for abstracts is Feb 24th 2013, with registration costing from €200-300 for students and academics.

3rd Cell Cycle and Cancer Conference France 2013

New Paper Published : Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells

Link Posted on Updated on

I am pleased to announce that the latest publication for the lab is now available online at PLoS ONE [Link][PDF].

Below are some additional videos of the Chromotek HeLa cell line [link] undergoing cell division, with the Imaris Dot quantification overlaid, along with a instructional video on how to perform the dot quantification analysis using Imaris Software.