HeLa SILAC conditions

Grow cells in DMEM (Sigma #D9443) without L-Arg, L-Leu, L-Lys ! This DMEM is low glucose 1000mg/L so you will need to add and extra 3000mg/L to make it unto 4000mg/L in total. Thus 15ml of the 10% Glucose solution is needed for 500ml of DMEM. Need to add 50ml of Mass Spec Grade Serum […]

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New Paper Published : Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells

I am pleased to announce that the latest publication for the lab is now available online at PLoS ONE [Link][PDF]. Below are some additional videos of the Chromotek HeLa cell line [link] undergoing cell division, with the Imaris Dot quantification overlaid, along with a instructional video on how to perform the dot quantification analysis using […]

Read More New Paper Published : Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells