Great news our latest publication “Global phosphoproteomic mapping of early mitotic exit in human cells identifies novel substrate dephosphorylation motifs” has been accepted by the top Proteomics Journal Molecular & Cellular Proteomics. You can currently download the unformatted version for free here [link] And here is an still image from the paper showing live HeLa […]Read More Our Latest Publication Accepted and Now Online!
General Guidelines These guides are written primarily for HeLa cells, but it should be possible to extend these to other cell lines with a bit of optimisation. 1) Make sure your cells are happy ! 2) Aim for around 75-85% confluence at the time of release. 3) Try and keep everything close to 37ºC, including […]Read More How to Synchronise Mammalian Cells in Culture
Grow cells in DMEM (Sigma #D9443) without L-Arg, L-Leu, L-Lys ! This DMEM is low glucose 1000mg/L so you will need to add and extra 3000mg/L to make it unto 4000mg/L in total. Thus 15ml of the 10% Glucose solution is needed for 500ml of DMEM. Need to add 50ml of Mass Spec Grade Serum […]Read More HeLa SILAC conditions
I am pleased to announce that the latest publication for the lab is now available online at PLoS ONE [Link][PDF]. Below are some additional videos of the Chromotek HeLa cell line [link] undergoing cell division, with the Imaris Dot quantification overlaid, along with a instructional video on how to perform the dot quantification analysis using […]Read More New Paper Published : Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells