Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image manipulationRead More User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use Thresholds in Image J, and here are a few that are well worth checking out [Link1][Link2]. Below are some of the Basic Steps for using Thresholds: Open your image and […]Read More Using Thresholds to Measure and Quantify Cells in Image J
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or […]Read More Using ImageJ to Measure Cell Fluorescence
Here is a recent talk I gave to some members of the public at the Garvan Institute of Medical Research. It is a very general and simple over-view of explaining 1) how cells in your body proliferate, 2) how this goes wrong in cancer, 3) the challenges we are facing in treating and killing cancer, and […]Read More Public Talk “Killing Cancer One Cell at a Time ” now on YouTube
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]Read More Immunofluorescence Guide
Great news, Cell Division Lab will be at the Sydney Light Optical Users Meeting, hosted by Dr Pamela Young at Sydney University, this Thursday (24th of July). I will be presenting a short seminar on “Imaging and Analysing Cell Division”. If you would like to attend please contact Pamela asap. Her details are below! Hope […]Read More We will be at the Sydney Light Optical Users Meeting on July 24th 2014
Some more good news to coincide with today’s official release of our manuscript, one of our images has been chosen to be the feature image on the front cover. It’s a great honour, one that I am very proud of, and is the first time I have ever had a front cover ! You can […]Read More Were the front cover feature image on this months issue of Cell Cycle !
Here is a slide that I made and often use at the beginning of my talks to briefly explain what mitosis is. The images are taken from a live cell that we have labelled with fluorescent probes to highlight the DNA (red) and microtubules (green). Enjoy !Read More A brief introduction to Mitosis
Here is one of the images that we took using a Leica SP8 confocal microscope this week in the lab. It is a 3D image of a HeLa cell that has completely stuffed up mitosis (undergone mitotic catastrophe). It has separated whole chromosomes randomly into 2 daughter cells instead of separating the two identical chromatids in […]Read More Cell Image of the Week – Mitotic Catastrophe
Great news for those who cannot afford the very price commercial alternatives for 3D analysis of microscope images. You can download the software here And you can read the original research article published in Nature Protocols hereRead More Vaa3D: New FREE open source software for 3D image analysis !