In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse and track individual cells to generate single cell fate maps. Note, unless you are also planning on quantifying fluorescent intensity data in your movies, I strongly suggest converting all of your image […]Read More How to analyse single cells time-lapse movies using Fiji and/or Photoshop
Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image manipulationRead More User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator
8th Garvan Signalling Symposium Date: Monday 31st of October and Tuesday 1st of November 2016 Venue: The Garvan Institute of Medical Research, Sydney Registration – Abstract submission to 15th of September 2016 The Garvan International Signalling Symposium is a premier meeting focused on the mechanisms of signal transduction. It began as a small meeting organised by […]Read More 8th Garvan Signalling Symposium – Registration Now Open !!!
Great news we are currently looking for a new honours student for 2016. The title of the project is “Developing novel biosensors to monitor DNA damage in cancer cells”. Its a very exciting new project incorporating cutting edge microscopy and fluorescent biosensors. If you think you have what it takes and are interested please feel […]Read More Position available: 2016 Honours Student Project in our Lab
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use Thresholds in Image J, and here are a few that are well worth checking out [Link1][Link2]. Below are some of the Basic Steps for using Thresholds: Open your image and […]Read More Using Thresholds to Measure and Quantify Cells in Image J
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or […]Read More Using ImageJ to Measure Cell Fluorescence
Great news our latest publication “Global phosphoproteomic mapping of early mitotic exit in human cells identifies novel substrate dephosphorylation motifs” has been accepted by the top Proteomics Journal Molecular & Cellular Proteomics. You can currently download the unformatted version for free here [link] And here is an still image from the paper showing live HeLa […]Read More Our Latest Publication Accepted and Now Online!
Great news, we recently published a collaborative paper in the journal Cell Division with the Lab of Dr Liz Caldon here at the Garvan. The title of the paper is “Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells”, and you can find it online here It also marked the […]Read More Congratulation to Sam our PhD student on his 1st Author Publication!
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]Read More Immunofluorescence Guide
Great news, Cell Division Lab will be at the Sydney Light Optical Users Meeting, hosted by Dr Pamela Young at Sydney University, this Thursday (24th of July). I will be presenting a short seminar on “Imaging and Analysing Cell Division”. If you would like to attend please contact Pamela asap. Her details are below! Hope […]Read More We will be at the Sydney Light Optical Users Meeting on July 24th 2014