How to analyse single cells time-lapse movies using Fiji and/or Photoshop

In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse and track individual cells to generate single cell fate maps. Note, unless you are also planning on quantifying fluorescent intensity data in your movies, I strongly suggest converting all of your image […]

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User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator

Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image manipulation

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8th Garvan Signalling Symposium – Registration Now Open !!!

8th Garvan Signalling Symposium  Date: Monday 31st of October and Tuesday 1st of November 2016 Venue: The Garvan Institute of Medical Research, Sydney Registration – Abstract submission to 15th of September 2016  The Garvan International Signalling Symposium is a premier meeting focused on the mechanisms of signal transduction. It began as a small meeting organised by […]

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Immunofluorescence Guide

The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce […]

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