User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator

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Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication.

PDF Download: Guide to FIJI-Photoshop Image manipulation


We will be at the Sydney Light Optical Users Meeting on July 24th 2014

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Great news, Cell Division Lab will be at the  Sydney Light Optical Users Meeting, hosted by Dr Pamela Young at Sydney University, this Thursday (24th of July).

I will be presenting a short seminar on “Imaging and Analysing Cell Division”.

If you would like to attend please contact Pamela asap. Her details are below!

Hope to see you there !

Sydney Light Optical Users Meeting July 2014

A brief Intro to Greatwall Kinase…The King of Mitosis

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Our favourite protein in the lab is Greatwall kinase. It was first discovered in 2004 to be critical for cell division in fruit flies (1,2) . The trail then went cold for a few years as to its exact function, but in 2009, while I was working as a post-doc in France, I was fortunate enough to be in the lab that uncovered its exciting mode of action. For cells to get into mitosis they must activate a key protein called cyclin dependent kinase 1 (Cdk1). I like to think of this as the accelerator in a car. So to get moving cells push on the gas!
And conversely to get out of mitosis you need to hit the brakes. These brakes are the phosphatases which reverse the action of kinases like Cdk1. That’s great but what is missing from this equation?
Well like any car it’s pretty useless without a driver to co-ordinate the accelerator and brakes. And this is where Greatwall (Gwl for short) comes in. It makes sure that when Cdk1 (accelerator) turns on that the breaks get turned off and vice versa (3,4). Without Gwl the cell gets into a lot of trouble very fast, which you can see in the image below. Here I depleted the human version of Gwl (a gene called MASTL) and watched what happened as cells tried to undergo mitosis (5). As you can see they don’t do a very good job… the result is cells fail to divide correctly, resulting in multiple defects and often cell death.

Gwl Figure

I hope you enjoyed part one of my feature on Gwl, and in part 2 I will into more details about this amazing and exciting new protein.


1. Bettencourt-Dias, M. et al. Genome-wide survey of protein kinases required for cell cycle progression. Nature 432, 980–987 (2004).

2. Yu, J. et al. Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in Drosophila. J Cell Biol 164, 487–492 (2004). [Link]

3. Vigneron, S. et al. Greatwall maintains mitosis through regulation of PP2A. EMBO J 28, 2786–2793 (2009). [Link]

4. Lorca, T. et al. Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycle. J Cell Sci 123, 2281–2291 (2010). [Link]

5. Burgess, A. et al. Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance. Proc Natl Acad Sci USA 107, 12564–12569 (2010). [Link]


Vaa3D: New FREE open source software for 3D image analysis !

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Great news for those who cannot afford the very price commercial alternatives for 3D  analysis of microscope images.
You can download the software here
And you can read the original research article published in Nature Protocols here



MitoticLabs Vimeo Channel

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MitoticLabs Vimeo Channel

Our lab now has its own Vimeo Channel where we will post videos and how to guides.

Check it out and let us know what you think